Environmental Enterprises USA, Inc.

Aquatic Toxicity Testing

Fresh Water Tests

Acute Static-Renewal EPA-821-R-02-012

EPA Method 2021, EPA Method 2000, EPA Method 2002, etc.

  • 24-Hour Definitive Test, Method 2000 (Pimephales promelas)
  • 24-Hour Definitive Test, Method 2002 (Ceriodaphnia dubia)
  • 24-Hour Definitive Test, Method 2021 (Daphnia pulex)
  • 48-Hour Definitive Test, Method 2000 (Pimephales promelas)
  • 48-Hour Definitive Test, Method 2002 (Ceriodaphnia dubia)
  • 48-Hour Definitive Test, Method 2021 (Daphnia pulex)
  • 96-Hour Definitive Test, Method 2000 (Pimephales promelas)
  • 96-Hour Definitive Test, Method 2002 (Ceriodaphnia dubia)

Seven-day Chronic Static-Renewal EPA-821-R-02-013

  • Survival and Reproduction Test, Method 1002 (Ceriodaphnia dubia)

Seven-day Chronic Static-Renewal EPA-821-R-02-013

EPA Method 1002 and EPA Method 1000

  • Larval Survival and Growth Test, Method 1000 (Pimephales promelas)

Other species and test information is available



Common names for the organisms listed above:

  • Pimephales promelas - carpita cabezona[Spanish], fathead minnow, tête-de-boule[French]
  • Ceriodaphnia dubia - water flea
  • Daphnia pulex - water flea

Common test names for these tests:

  • Freshwater toxicity test
  • Freshwater toxicity testing
  • Freshwater toxicity tests
  • Water toxicity testing
Pimephales promelas
Ceriodaphnia dubia