© 2017 Bee Seen Designs

Environmental Enterprises USA, Inc.

Common Names for these tests:  Freshwater Toxicity or Water Toxicity

Freshwater Testing Methods

Whole Effluent Toxicity (WET) tests were developed as a tool to evaluate the potential harmful effects of effluent discharged into surface water. Carefully chosen indicator organisms are exposed to whole effluent and/or effluent dilutions for a specified period of time to observe the effluent's effect on the organisms and its potential to effect organisms in the receiving stream. Test methods and duration of the tests are specified in the discharger's permit. 

Whether you are a waste water treatment plant, chemical plant, refinery, or product manufacturer, contact us to discuss your permit or research needs.

EPA - 821-R-02-012, Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater Marine Organisms.

24, 48 & 96 Hour Definitive Tests:

Method 2002, Ceriodaphnia dubia or "Water Flea"

Method 2021, Daphnia pulex or "Water Flea"

Method 2000, Pimephales promelas or "Fathead Minnow"

EPA - 821-R-02-013, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

7-Day Chronic Toxicity Tests:

Method 1002, Larval Survival and Reproduction Test, Ceriodaphnia dubia

Method 1000, Survival and Growth Test, Pimephales promelas

LC50, IC25, and NOEC Statistical Analyses Available

All organisms for primary test methods are cultured in-house, providing immediate availability as well as exceptional quality organisms. 

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EE USA's staff are thoroughly and annually trained to perform tests according to Standard Operating Procedures developed following EPA methodology and NELAP standards. They have the skills and experience to develop methods for special projects as well as complete primary test methods.